Integrated analysis of lncRNA–miRNA–mRNA ceRNA network and the potential prognosis indicators in sarcomas

Abstract Background Competitive endogenous RNA (ceRNA) networks have revealed a new mechanism of interaction between RNAs, and play crucial roles in multiple biological processes development neoplasms. They might serve as diagnostic prognosis markers well therapeutic targets. Methods In this work, we identified differentially expressed mRNAs (DEGs), lncRNAs (DELs) miRNAs (DEMs) sarcomas by comparing the gene expression profiles sarcoma normal muscle samples Gene Expression Omnibus (GEO) datasets. ontology (GO) Kyoto encyclopedia genes genomes (KEGG) pathway enrichment analyses were applied to investigate primary functions overlapped DEGs. Then, lncRNA-miRNA miRNA-mRNA interactions predicted, ceRNA regulatory network was constructed using Cytoscape software. addition, protein–protein (PPI) survival analysis performed. Results A total 1296 DEGs combining GO KEGG analyses, 338 DELs discovered after probes reannotated, 36 DEMs ascertained through intersecting two different sets. Further, target prediction, lncRNA–miRNA–mRNA that contained 113 mRNAs, 69 29 constructed. The PPI six most significant hub proteins. Survival seven four one lncRNA associated with overall patients. Conclusions Overall, sarcomas, which provide insights for further research on molecular potential biomarkers.